The expression of both genes was upregulated under different hormone treatments, which indicated that the two genes positively regulate the hormone response. Here, we presume that the two genes are involved in the formation of primary cell walls and that AmCesA2 is also involved in the formation of secondary cell walls.
#De novo protein sequence analysis full#
The two genes responded to GA₃, 6-BA, and MeJA treatments, and the response to GA₃ was relatively strong. The de novo antibody protein sequencing platform offers a mass spectrometry solution that accurately obtains the full amino acid sequence without requiring the. Real-time RT-PCR showed that both genes were widely expressed in roots, stems, and leaves, but AmCesA2 was more highly expressed in stems. Southern blot analysis showed that multiple copies of AmCesA1 were present in the Acacia mangium genome, but only a single copy of AmCesA2 was present. Today, analysis by a tandem mass spectrometer is a more common method to solve the sequencing of peptides. In the old days, this was accomplished by the Edman degradation procedure. Knowing the amino acid sequence of peptides from a protein digest is essential for studying the biological function of the protein. AmCesA2 had high degrees of similarity with Leucaena leucocephala LlCesA7 and LlCesA8. In mass spectrometry, de novo peptide sequencing is the method in which a peptide amino acid sequence is determined from tandem mass spectrometry. Cluster analysis showed that AmCesA1 had high degrees of similarity with Glycine max GmCesA1 and Arachis duranensis AdCesA1. AmCesA1 was determined to have six transmembrane regions, and AmCesA2 was determined to have eight transmembrane regions. In silico analysis revealed that AmCesA1 cDNA was 3793 bp in size, had a 3249 bp ORF, and encoded a 1082 amino acid protein AmCesA2 cDNA was 3743 bp in size, had a 3228 bp ORF, and encoded a 1075 amino acid protein.
In this study, two cellulose synthase genes, AmCesA1 and AmCesA2, were cloned from Acacia mangium using transcriptome de novo sequencing analysis and RACE. 85 ISSN: 1614-2942 Subject: Acacia mangium, Arachis duranensis, Glycine max, Leucaena leucocephala, Southern blotting, amino acids, cell walls, cellulose, cellulose synthase, cluster analysis, complementary DNA, fiber quality, gene expression regulation, genes, gibberellic acid, leaves, open reading frames, quantitative polymerase chain reaction, reverse transcriptase polymerase chain reaction, roots, sequence analysis, stems, transcriptome, wood fibers Abstract: Cellulose synthase (CesA) plays a major regulatory role in the cellulose synthesis pathway in plants and is an important factor in controlling wood fiber quality and yield. Cloning and analysis of cellulose synthase genes (CesA) in Acacia mangium Author: Jian Ren, Yuqing Yin, Dian Chen, Yong Wang Source: Tree genetics & genomes 2018 v.14 no.6 pp. QUARK is a computer algorithm for ab initio protein structure prediction and protein peptide folding, which aims to construct the correct protein 3D model. De novo analysis of protein N-terminal sequence is important for identification of N-terminal proteolytic processing such as N-terminal methionine or signal peptide removal, or for the genome annotation of uncharacterized proteins.
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